Show cover of SLAS Technology Authors Talk Tech

SLAS Technology Authors Talk Tech

We invite you to "get behind the science" with SLAS Technology Editorial Board Member and Podcast Editor Dave Pechter, M.S.M.E. (PerkinElmer, Cambridge, MA) and hear from our featured SLAS Technology authors! This podcast series is a chance for readers to meet the people behind the journal science and hear directly from them about their work, their motivations, as well as the context and potential impact of their work. Watch for a new featured author interview with each published issue!

Tracks

Behind the Special Issue: Single Cell Analysis Technologies
Behind the Special Issue: Join podcast host David Pechter as he invites Joseph de Rutte (Partillion Bioscience) to discuss the paper: “Sorting single-cell microcarriers using commercial flow cytometers”. This research was featured in the recent SLAS Technology special issue, Single Cell Analysis Technologies. Listen in as they discuss the unique formation of microscopic, bowl-shaped containers they call nanovials, and the applications of these cell carriers in flow cytometry for functional single-cell assays. Dr. Joe de Rutte received his B.S. and M.S. in Mechanical Engineering from the University of California, Santa Barbara in 2014 and 2016 and received a Ph.D. in Bioengineering from the University of California, Los Angeles in 2020. He co-founded Partillion Bioscience in 2020 based on his Ph.D. research and currently leads the company as President. Dr. de Rutte was awarded the 2020 Society of Laboratory Automation and Screening Innovation Award in recognition of the impact of his “Lab on a Particle” work.For more on applications of single cell analysis technologies, read the full open access special issue: Single Cell Analysis TechnologiesVisit https://www.slas.org/publications/slas-technology/ for more information about SLAS and its journals.
16:24 06/15/2022
Assay Guidance Manual: Technologies that Matter
Join SLAS Podcast Host Dave Pechter as he welcomes R. Graham Cooks, Ph.D. (Purdue University) and Nicolás M. Morato (Purdue University) to discuss their research, featured in the latest Issue of SLAS Technology. They are joined by Nathan P. Coussens, Ph.D. (Frederick National Laboratory for Cancer Research), guest editor of this special issue.  Together, they discuss the article “Automated High-Throughput System Combining Small-Scale Synthesis with Bioassays and Reaction Screening”. This work explores the application of desorption electrospray ionization (DESI) for high-throughput synthetic chemistry, chemical reaction screening, and label-free biological assays.This article is part of the December Special Issue: “Assay Guidance Manual for Drug Discovery: Technologies that Matter”, part of a joint collaboration between the Assay Guidance Manual and SLAS. The goal of these combined publications is to tackle the crisis of reproducibility in biomedical science by utilizing rigorous data analysis, strong reporting standards and robust assays. The Assay Guidance Manual is a free and publicly available eBook of best practices for the design, development, and implementation of robust assays in preclinical research. For more information about the journal, visit the SLAS Technology website or contact the SLAS publishing office at publishing@slas.org 
20:09 12/08/2021
In Conversation: SLAS Editors on Open Access
Join Jill Hronek, SLAS Director of Marketing and Communications, and Ally Jump, SLAS Publishing Manager, as they speak with SLAS Discovery and SLAS Technology’s respective Editors-in-Chief, Robert M. Campbell, Ph.D., (Twentyeight-Seven Therapeutics, Boston, MA USA) and Edward Kai-Hua Chow, Ph.D., (National University of Singapore, Singapore) about SLAS’s new Open Access model for the Society’s two peer-reviewed journals.  Beginning January 1, 2022 both SLAS Discovery and SLAS Technology will be available Open Access and through a new publisher, Elsevier. Tune in to have your questions answered about all things publishing and open access and hear what other exciting things the SLAS publishing team has in store for the new year.Check out our SLAS Open Access FAQs and Elsevier Open Access Agreements to see if your institution covers publishing costs!
13:38 10/27/2021
Low-Cost 3D Bioprinters: State of The Market and Observed Future Trends
Recent innovations and accelerating market availability have led to tremendous growth in demand for affordable 3D bioprinter hardware. Podcast host David Pechter invites SLAS Technology August cover authors Roman Voronov and Anh Tong of the New Jersey Institute of Technology to discuss their review of the market, and their predictions about where it is going.This month's SLAS Technology cover article provides essential insights to researchers entering a market flooded with different types of low-cost bioprinter models. Click here to read the full review!
15:59 08/25/2021
Discussion on SLAS Featured Talk: Harnessing Synthetic Biology and Deep Learning to Fight Pathogens
Podcast Editor David Pechter hosts Jim Collins of the MIT Department of Biological Engineering to discuss his talk on the harnessing of deep learning to fight pathogens.  The future of medicines using synthetic biology is a rapidly expanding field, with applications including engineered diagnostics, gene and cell therapies, and the deployment of mRNA vaccines to fight the COVID-19 pandemic. The application of deep learning is accelerating this research, facilitating rapid candidate identification for novel therapies.
16:21 07/28/2021
Special Collection: AI in Process Automation
Volume 26 Issue 1, February 2021Dave Pechter discusses the February SLAS Technology Special Collection AI in Process Automation with Guest Editor Cenk Undey (Amgen), which includes the following papers:Artificial Intelligence Effecting a Paradigm Shift in Drug Development by Masturah Bte Mohd Abdul RashidArtificial Intelligence (AI) to the Rescue: Deploying Machine Learning to Bridge the Biorelevance Gap in Antioxidant Assays by Sunday Olakunle Idowu and Amos Akintayo FatokunDeep Learning and Computer Vision Strategies for Automated Gene Editing with a Single-Cell Electroporation Platform by Cesar Patino, Prithvijit Mukherjee, Vincent Lemaitre, et al.
12:34 01/13/2021
Special Collection: Advances in Technology to Address COVID-19
Volume 25 Issue 6, December 2020Dave Pechter discusses the December SLAS Technology Special Collection Advances in Technology to Address COVID-19 with Associate Editor Pak Kin Wong (The Pennsylvania State University), which includes Professor Wong's review Advances in Viral Diagnostic Technologies for Combating COVID-19 and Future Pandemics.
09:24 11/18/2020
Role of Digital Microfluidics in Enabling Access to Laboratory Automation and Making Biology Programmable
Volume 25 Issue 5, October 2020Dave Pechter discusses with Varun Bhaskar Kothamachu, Ph.D. (Head of Product, Digi.Bio) & Sabrina Zaini (Head of Operations, Digi.Bio) their featured research article, "Role of Digital Microfluidics in Enabling Access to Laboratory Automation and Making Biology Programmable."
13:54 09/14/2020
Special Issue: Carbohydrate Structure Analysis: Methods and Applications
SLAS Technology 25.4 August 2020
13:28 07/20/2020
Traceable Impedance-Based Dispensing and Cloning of Living Single Cells and Impedance-Based Single-Cell Pipetting
Volume 25 Issue 3, June 2020Dave Pechter discusses with Georges Muller & Yann Barrandon their two featured research articles, "Traceable Impedance-Based Dispensing and Cloning of Living Single Cells" and "Impedance-Based Single-Cell Pipetting."Traceable Impedance-Based Dispensing and Cloning of Living Single Cells: Single-cell cloning is essential in stem cell biology, cancer research, and biotechnology. Regulatory agencies now require an indisputable proof of clonality that current technologies do not readily provide. Here, we report a one-step cloning method using an engineered pipet combined with an impedance-based sensing tip. This technology permits the efficient and traceable isolation of living cells, stem cells, and cancer stem cells that can be individually expanded in culture and transplanted.Impedance-Based Single-Cell Pipetting: Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here, we report the modeling, designing, and testing of a disposable pipet tip integrating a cell sensor based on the Coulter principle. We investigate, test, and discuss the effects of design parameters on the sensor performances with an analytical model. We also describe a system that enables the dispensing of single cells using an instrumented pipet coupled with the sensing tip. Most importantly, this system allows the recording of an impedance trace to be used as proof of single-cell isolation. We assess the performances of the system with beads and cells. Finally, we show that the electrical detection has no effect on cell viability.
13:24 06/01/2020
CURATE.AI: Optimizing Personalized Medicine with Artificial Intelligence
Volume 25 Issue 2, April 2020Dave Pechter discusses with Agata Blasiak & Theodore Kee regarding their article, "CURATE.AI: Optimizing Personalized Medicine with Artificial Intelligence." The clinical team attending to a patient upon a diagnosis is faced with two main questions: what treatment, and at what dose? Clinical trials’ results provide the basis for guidance and support for official protocols that clinicians use to base their decisions upon. However, individuals rarely demonstrate the reported response from relevant clinical trials, often the average from a group representing a population or subpopulation. The decision complexity increases with combination treatments where drugs administered together can interact with each other, which is often the case. Additionally, the individual’s response to the treatment varies over time with the changes in his or her condition, whether via the indication or physiology. In practice, the drug and the dose selection depend greatly on the medical protocol of the healthcare provider and the medical team’s experience. As such, the results are inherently varied and often suboptimal. Big data approaches have emerged as an excellent decision-making support tool, but their application is limited by multiple challenges, the main one being the availability of sufficiently big datasets with good quality, representative information. An alternative approach—phenotypic personalized medicine (PPM)—finds an appropriate drug combination (quadratic phenotypic optimization platform [QPOP]) and an appropriate dosing strategy over time (CURATE.AI) based on small data collected exclusively from the treated individual. PPM-based approaches have demonstrated superior results over the current standard of care. The side effects are limited while the desired output is maximized, which directly translates into improving the length and quality of individuals’ lives.
19:54 04/01/2020
Special Issue: Flexible Analytical Devices for Point-of-Care Testing
Volume 25 Issue 1, February 2020Dave Pechter discusses with Hideaki Tsutsui & Peter Lillehoj regarding February's Special Issue. Over the last decade, flexible analytical devices have received considerable attention in both academia and industry. Compared to conventional analytical devices which are generally made from rigid materials, such as silicon, glass, and plastics, flexible devices offer several unique advantages, such as simplified fabrication, lower costs, enhanced portability and disposability, and compliance to curved or deforming surfaces. For these reasons, flexible analytical devices are well suited for many diagnostic applications, including wearable and in vivo sensing, and point-of-care testing for disease detection and health monitoring. This special issue showcases a comprehensive review and exciting original research on topics ranging from wearable sensors for human motion monitoring and disease diagnosis, flexible electrochemical sensor arrays for human cell culture monitoring, paper-based sensors and immunoassays for diagnostic testing, a paper-based biological solar cell for power generation and storage, and a 3D printing strategy for rapid prototyping of flexible microfluidic devices.
11:52 02/01/2020
Automated System for Small-Population Single-Particle Processing Enabled by Exclusive Liquid Repellency
Volume 24 Issue 6, December 2019Dave Pechter discusses with Chao Li the article, "Automated System for Small-Population Single-Particle Processing Enabled by Exclusive Liquid Repellency." Lossless processing and culture of rare cells (e.g., circulating tumor cells, drug-persistent microorganisms) at single-cell level is of great significance in understanding the heterogeneity of carcinogenesis or human pathogenesis caused by microbial infection. Current single-cell isolation techniques like fluorescence-activated cell sorting (FACS) require relatively large sample volume and cell number to work with, and inflict sample loss and reduced cell viability from the processing. While microfluidic and single-cell printing techniques allow the handling of minute amounts of cellular samples, they either come with limited physical access to the sample of interest due to the closed-channel design (e.g., droplet microfluidics) or sample loss during aspiration, transfer, and sample retrieval from culture.Recently, we reported an extreme wettability phenomenon, named exclusive liquid repellency or ELR. ELR is observed in solid-liquid-liquid three phase systems, where a solid surface shows complete repellency to a liquid (with Young’s contact angle, CA = 180o) when exposed to a second immiscible liquid. This phenomenon is observed when a particular thermodynamic boundary condition is satisfied (i.e., γS/Lcp + γLdp/Lcp ≤ γS/Ldp, where γ - interfacial tension, S - solid, Lcp - liquid of continuous phase, and Ldp - liquid of dispersed phase). Neither surfactant nor flow condition is required, e.g., compared with droplet microfluidics. ELR enables additional fluidic control, robust on-chip cell culture, and improved processing of rare cell samples in open aqueous fluid under oil. ELR is distinct from other liquid repellent systems with CA < 180o (i.e., non-ELR), showing no compromise of liquid adhesion on solid surfaces and enabling unique applications.In this work, we developed an automated platform using ELR microdrops for lossless single-particle (or single-cell) isolation, identification, and retrieval. It features the combined use of a robotic liquid handler, an automated microscopic imaging system, and real-time image-processing software for single-particle identification. The automated ELR technique enables rapid, hands-free, and robust isolation of microdrop-encapsulated rare cellular samples, and further on-chip cell culture or down-stream analysis (e.g., RNA extraction and RT-qPCR).
10:07 12/01/2019
Special Issue: Engineering Innovations for Fundamental Biology and Translational Medicine
SLAS Technology 24.5 October 2019Professors at Johns Hopkins University, Claire Hur and Deok-Ho Kim, discuss their special issue, "Engineering Innovations for Fundamental Biology and Translational Medicine."In this special issue of SLAS Technology, the editors showcase reviews and original research reports addressing the accuracy in diagnostic and prognostic tests performed on the patient-derived specimen, an emerging technology demand for personalized medicine.Soojung Claire Hur, Ph.D., is Clare Boothe Luce Assistant Professor in the Department of Mechanical Engineering at Johns Hopkins Whiting School of Engineering, and an expert in microfluidics. Deok-Ho Kim, Ph.D., is faculty in the Department of Biomedical Engineering and Department of Medicine at the Johns Hopkins University School of Medicine.Topics include the role of microfluidic technology in patient-specific information collection and endeavors to integrate innovative technologies with existing clinical workflows through automation and miniaturization for expedited translation.
10:35 10/01/2019
Technologies for the Directed Evolution of Cell Therapies
Volume 24 Issue 4, August 2019UCLA Professor, Dino Di Carlo, discusses his review paper, "Technologies for the Directed Evolution of Cell Therapies." The next generation of therapies is moving beyond the use of small molecules and proteins to using whole cells. Compared with the interactions of small-molecule drugs with biomolecules, which can largely be understood through chemistry, cell therapies act in a chemical and physical world and can actively adapt to that world, amplifying complexity but also the potential for truly breakthrough impact. Although there has been success in introducing targeting proteins into cells to achieve a therapeutic effect, for example, chimeric antigen receptor (CAR) T cells, our ability to engineer cells is generally limited to introducing proteins, but not modulating large-scale traits or structures of cellular “machines,” which play critical roles in disease. Example traits include the ability to secrete compounds, deform through tissue, adhere to surrounding cells, apply force to phagocytose targets, or move through extracellular matrix. There is an opportunity to increase the efficacy of cell therapies through the use of quantitative automation tools, to analyze, sort, and select rare cells with beneficial traits. Combined with methods of genetic or epigenetic mutagenesis to create diversity, such approaches can enable the directed cellular evolution of new therapeutically optimal populations of cells and uncover genetic underpinnings of these optimal traits.
11:48 08/01/2019
Special Collection: Advances in 3D and Organoid Cell Culture
SLAS Technology 24.1 February 2019Guest Editor Richard M. Eglen of Corning Life Sciences (Tewksbury, MA, USA) talks about a new collection of reviews and original research reports that illustrate how the combination of human iPS cells and 3D cell culture technology provide powerful new approaches to the development of novel and more effective therapies. Free access to the published collection is sponsored by Corning Life Sciences.
13:56 02/01/2019
Improved Fluorescence Methods for High-Throughput Protein Formulation Screening
SLAS Technology 23.6 December 2018University of Kansas researchers Yangjie Wei and Nicholas Larson talk about how they used a steady-state/lifetime fluorescence-based, high-throughput platform to develop a general workflow for direct formulation optimization under analytically challenging but commercially relevant conditions.
06:37 12/01/2018
Special Issue: The Internet of Things (IoT) in the Life Sciences Laboratory
SLAS Technology 23.5 October 2018Guest Editor James M. Gill, II, Ph.D., of BFL Consulting (Madison, CT) talks about how the October 2018 issue of SLAS Technology showcases life sciences researchers who are pioneering the use of powerful yet accessible and low-cost IoT technologies in their laboratories. Ten articles illustrate how IoT is being used to optimize factors such as throughput, cost, uptime and result quality.
07:46 10/01/2018
Continuous and Quantitative Purification of T-Cell Subsets for Cell Therapy Manufacturing Using Magnetic Ratcheting Cytometry
SLAS Technology 23.4 August 2018UCLA’s Coleman Murray, Ph.D., talks about his contribution to the August 2018 special issue of SLAS Technology on Enabling Technology in Cell-Based Therapies by guest editor Chris Puleo and his associates at General Electric Global Research. Murray’s article illustrates an important example of how new technologies are changing automation and processing in cell therapy manufacturing.
07:11 08/01/2018
Special Issue: Quantitative Imaging in Life Sciences and Biomedical Research
Volume 23 Issue 3, June 2018Guest Editor Anand D. Jeyasekharan, Ph.D., of the Cancer Science Institute of Singapore, National University of Singapore, Centre for Translational Medicine, talks about the SLAS Technology special issue on Quantitative Imaging in Life Sciences and Biomedical Research. His insight and the articles in this special issue offer a meaningful glimpse into the potential and applicability of quantitative imaging as life sciences and biomedical research move into an era of big data and high-content experiments.
07:01 06/01/2018
Fully Automated Pipetting Sorting System for Different Morphological Phenotypes of Zebrafish Embryos
Volume 23 Issue 2, April 2018Christian Pylatiuk, Prof. Dr. med., of the Karlsruhe Institute of Technology, Institute for Automation and Applied Informatics in Eggenstein-Leopoldshafen, Germany, discusses his new article, which describes a zebrafish embryo sortying system with two cameras and image processing based on template-matching algorithms.
09:51 04/01/2018
Fast and Label-Free Isolation of Circulating Tumor Cells from Blood: From a Research Microfluidic Platform to an Automated Fluidic Instrument, VTX-1 Liquid Biopsy System
Volume 23 Issue 1, February 2018Meet Elodie Sollier-Christen of Vortex Bioscienes as she talks with SLAS Technology Podcast Editor David Pechter about the groundbreaking technology that earned her the 2017 SLAS Innovation Award.
15:33 02/01/2018
Emerging Microtechnologies and Automated Systems for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
SLAS Technology 22.6 December 2017Listen to author Weian Zhao of the University of California, Irvine, as he talks about his new article, which summarizes and analyzes representative emerging micro- and nanotechnologies and automated systems for bacterial ID/AST, including phenotypic and molecular methods; discusses representative point-of-care systems that integrate sample processing, fluid handling, and detection for rapid bacterial ID/AST; and highlights major challenges and potential future endeavors toward improving clinical outcomes.
10:31 12/01/2017
Heart-on-a-Chip: An Investigation of the Influence of Static and Perfusion Conditions on Cardiac (H9C2) Cell Proliferation, Morphology, and Alignment
SLAS Technology 22.5 October 2017Dr. Elzbieta Jastrzebska of Warsaw University of Technology (Poland) talks about how microsystems can be used to understand processes in heart tissue in detail and to test newly developed compounds applied in the treatment of cardiac diseases.
07:58 10/01/2017
Digital Assays
SLAS Technology 22.4 August 2017Dr. Amar Basu of Wayne University talks with SLAS Technology Podcasts Editor David Pechter about his in-depth, two-part review on digital assays (partitioning statistics and digital PCR; digital protein and cell assays).
16:54 08/01/2017
Making N-of-1 Medicine a Reality
SLAS Technology 22.3 June 2017Special issue guest editors Dean Ho, Ph.D. and Ali Zarrinpar, M.D., Ph.D. of the University of California, Los Angeles, talk about the state of personalized and precision medicine and the 11 reviews and reports that explore innovation from a cross section of the disciplines driving major advances in PPM.
12:55 06/01/2017
New Technologies for Rapid Bacterial Identification and Antibiotic Resistance Profiling
SLAS Technology 22.2 April 20172016 SLAS Innovation Award Winner Shana O. Kelly talks about new solutions for faster bacterial identification and analysis, and recent advances in managing infectious diseases by leveraging single-cell analysis, microfluidic concentration and detection strategies, and ultrasensitive readout mechanisms.
09:19 04/01/2017
The 2017 SLAS Technology Ten
SLAS Technology 22.1 February 2017From being able to move liquid with sound to dispense primary cells and DNA to microfluidic-based immunoassays, SLAS Technology Editor-in-Chief talks about the groundbreaking achievements reflected in the 2017 SLAS Technology Ten. This year’s highlighted papers push the envelope of what is possible with automation, integrating new functions into high-throughput sample preparation and screening to further drive efficiencies in research.
12:59 02/01/2017